High-resolution melting (HRM) analysis is a new and rapid method for the detection of mutations, in which PCR and mutation scanning are carried out simultaneously in a single procedure [ 17].
Any region that is found to potentially contain a mutation by scanning is then characterised by sequencing.
High-resolution melting (HRM) analysis is a very effective method for genotyping and mutation scanning that is usually performed just after PCR amplification (the "closed tube" format).
The set of exons selected for this mutation scanning analysis were drawn from cancer genes frequently mutated (PIK3CA, ERBB2, KRAS, TP53, EGFR, BRAF, GATA3, and FGFR3) in published reports, with a particular emphasis on breast, ovarian and endometrium cancers [5], [9], [27], [28], [29], [30], [31], [32].
Scan while the scanning is still good.
Mutation scanning with HRMA is based on the dissociation behaviour of DNA when exposed to an increasing temperature, in the presence of intercalating fluorescent dyes.
Mutation scanning in particular is reliant on heteroduplex formation that distorts the shape of the melting curve as compared with a normalised curve of a wild-type reference (Reed et al, 2007).
In many cases, particularly for mutation scanning methods, it is necessary to use a qualitative description to distinguish between a single normal state (negative result) and any number of mutated states (positive result).
Mutation scanning technology has been used to develop crop species with improved traits.
