Sentence examples for mutation scanning for from inspiring English sources

We previously validated mutation scanning for BRCA1 and 2 using high-resolution melting curve analysis (HRMCA).

In conclusion, CSCE is a robust technique with a high analytical sensitivity and specificity, and it can readily be used for mutation scanning of the large SACS gene.

The aim of this study was to assess the sensitivity and specificity of an inexpensive HRM analysis platform for mutation scanning of single-base variation in a range of tumor samples: frozen pediatric small rounded blue-cell tumors and paraffin-embedded tumors from breast, endometrium and ovarian cancers.

Using DHPLC for mutation scanning of all 65 exons of FBN1, initially no mutation was found in case 9.

This will provide further insight into the true sensitivity of HRM and applicability of the method for mutation scanning of the entire coding regions of BRCA1 and BRCA2.

We designed PCR amplicons for HRM mutation scanning of TP53 exons 5 to 8 and tested them with DNA from cell lines hemizygous or homozygous for known mutations.

As a result, mutation scanning of short amplicons becomes feasible on a standard real-time PCR instrument (not primarily designed for HRM) using SYBR Green I.

DHPLC mutation scanning of coding exons, splice junctions, and flanking intronic portions of SOS1 did not reveal any putative pathogenic mutation in the nine subjects with a diagnosis of CFCS and negative for mutations in BRAF, KRAS, MAP2K1, and MAP2K2.

For example, the performance specification for a hypothetical method for mutation scanning (>95% sensitivity for mutations in fragments <300 bp long and 25 60% G+C content) would not be applicable to a new test involving a 400-bp fragment or fragments with 70% G+C content.

The relatively low amount of SNPs in BRCA1 and BRCA2 make HRM a suitable mutation scanning technique for these genes.

We therefore evaluated the use of high resolution melting (HRM) as a rapid mutation scanning tool for TP53 in tumour samples.