Sentence examples for mutation scanning and from inspiring English sources
The development of accurate and inexpensive instruments that offer HRM capabilities, and new fluorescent dyes, make this method attractive for targeted mutation scanning and also germ line genotyping.
Initially developed for mutation scanning and genotyping studies [ 8- 12], high-resolution melting technology can be useful for the detection of methylation as well.
The recent application of HRM to mutation scanning and SNP genotyping as well as DNA methylation studies have been demonstrated [ 26- 31].
Molecular analysis for OI includes sequencing or mutation scanning and deletion/duplication testing for COL1A1 and COL1A2, followed by sequencing of recessive OI-related genes (see Table 1).
The HRM method is a scanning technique that enables mutation scanning and amplification to be performed readily, and in one step [ 2].
These assays are convenient, rely on endpoint analysis, improve the options for construction of multiplex assays, and are suitable for SNP genotyping, mutation scanning, and detection of DNA duplication or deletions.
This work provides a starting point for mutation scans and association studies on dogs with behavioural problems.
HRM analysis is the next generation of mutation scanning technology and can be cost-effective, requiring less time for results when compared to other scanning techniques.
Mutations in BRCA1 and BRCA2 have been detected with a variety of techniques including multiple mutation scanning methods and Sanger sequencing (reviewed in [ 28]).
The high cost of mutation scanning techniques and conventional 'Sanger' sequencing has limited systematic searches for rare variants (40), but the recent emergence of clonal sequencing technology (41, 42) makes such work more cost effective.
After a thorough study on the experimental conditions for FMCA, we demonstrated that these two types of probes enable FMCA to be used for mutation scanning, mutation identification and mutation genotyping and confer cross-platform compatibility on major real-time PCR instruments.
