To evaluate the complementation of the mutator phenotype, mutant frequencies and mutation rates were determined.
In addition, the mutation rates were surprisingly variable.
Mutation rates were calculated using total read numbers at sequence position.
PTEN mutation rates were higher in both msh2 (4.4 x 10-5) and mlh1 strains (2.3 x 10-5).
The lysine residues and mutation rates were typical, he said, and many swine flus seem unrelated because not enough pigs are tested each year.
The mutation rates were calculated by dividing number of 100% mutation shoots by the total number of all-types of mutated shoots.
As with M. smegmatis, low mutation rates were recovered by complementation with the wild-type S. coelicolor nucS gene (Fig. 2c, Supplementary Table 3).
Significantly, basal mutation rates were recovered when the nucS deletion was chromosomally complemented with the wild-type gene MSMEG_4923 (nucSSm) (Fig. 2a, Supplementary Table 1), confirming that inactivation of this gene was responsible for the high mutation rates observed.
The posterior distributions for mutation rates were 2.1 × 10−4 (±s.d. 1.02 × 10−4) for microsatellite DNA loci and 2.93 × 10−7 (±s.d. 1.49 × 10−7) for the mtDNA sequence (per site per year).
With the 16 pairs of primer combinations, 54 space-induced mutation sites were observed in 971-5, 86 in 972-4, and 5 in R955 compared to their controls, and the mutation rates were 4.3%, 6.9% and 0.4%, respectively.
Mutation rates were elevated for all of these strains, but the differences in rates among the strains were substantially reduced.
