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In all cases, a high selection pressure (but not an extreme one) combined with a moderately high mutation rate works best, while crossover provides some benefit but only on the less rugged landscapes.
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Also, it is important to understand how context affects mutation rates when working in the field of molecular phylogenetics.
The correlation between replication timing and mutation rate in this work raises the question why this relationship was not identified in previous experimental studies.
The proportions of reference sites and non-reference sites were set based on estimates from previous population-based study, which were several orders of magnitude larger than the mosaic mutation rate estimated in this work.
Given the intraspecific level, in addition to finding new evidence of transatlantic LDD of A. germinans that may contribute to its widespread distribution within the South American Atlantic basin, we observed partially discordant molecular variation patterns between high (microsatellites - [ 26]) and low (DTHK and ITS – present work) mutation rate markers for both A. germinans and A. schaueriana.
Tobacco, sunburns and charred food increase the mutation rate, and the immune system's work.
The cost and rate of TILLING is dependent partially on the quality of the DNA being screened (how reliably the reactions work) and the mutation rate (how many alleles are identified per pooled population).
A discordant result may be due to the different nature of the markers used in the different works (e.g. mutation rate, recombination, sex specific inheritance).
The mutation rate is selected based upon the existing work in the channel selection process.
Importantly, in our previous work [ 15], the mutation rate of locus 168 was higher in the caries-free group than in the caries-active group, and the mutation rate of locus 470 was higher in the caries-active group than in the caries-free group.
Previous work showed that the mutation rate at microsatellites increased with repeat unit length (Tran et al. 1997; Wierdl et al. 1997).
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