There was a great deal of variability among loci in mutation rate (Table 2).
To remove the effect of the mutation rate, we divided PSSs by the corresponding mutation rate (Table 6).
The isogenic SK1 strain EAY1062 [ lys2 :: InsE-A 14 (Nishant et al. 2008)] was used to measure the effect of mlh3 mutations on mutation rate (Table 3).
To characterize the hypermutator phenotype in PAHM4, the frequency of emergence of rifampicin-resistance was used to calculate the mutation rate (Table 1).
Molecularly, the serrated polyps were significantly more methylated and BRAF mutant as expected, and all polyp subtypes had a low KRAS mutation rate (Table 3).
Wild-type PAO1 had a mutant frequency of 4.9 x 10−9 and a mutation rate of 1.8 x 10−9 mutations/cell/generation, while a Δ mutS isogenic strain, PAOMS, showed a 2,000-fold 2,000-foldn mutatincrease (Table 1).
Produces a negative class of in silico passenger mutations by random nucleotide substitution in a library of expressed human mRNA transcripts from NCBI RefSeq, according to the distributions specified in the passenger mutation rate table (Supplementary Material ).
The estimates of post-split gene flow eastwards towards the White Sea (m1; ratio of immigration rate to mutation rate) were practically zero, whereas significant gene flow westwards to the NW Pacific was implied, amounting to m2 = 1 5, i.e. at a rate similar to the per-gene mutation rate (Table 4).
In a haploid strain with duplication of the POL3 gene, encoding a catalytic subunit of Pol δ, when one allele is the pol3-01 anotherther allele is the wild-type, there is 9.6-fold increase in Canr forward mutation rate and a 22-fold increase in frameshift mutation rate (Table 4).
Both rounds of the RT-PCRs for the assessment of transcript mutation rates (Table S2) and the first round of mutation enrichment RT-PCRs (RT-ePCRs) employed PfuUltra2 enzyme, a high fidelity Taq polymerase enzyme, following the recommended protocol (Stratagene).
