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We then used this relationship to predict the expected number of mutations at a site and then simulated data based on these expectations with and without additional variation in the mutation rate (details in the section Materials and Methods).

As demonstrated above, this allows for close fitting of complex marker behaviour (Fig. 1), and for an approximation of the distribution of fitness effects (Fig. 3), though at higher mutation rates, details of changes in the full haplotype distribution are missed.

Fourth-order Daubechies wavelet transformation was then conducted on the binned and normalized mutation rate (see below for normalization details) using Wolfram Mathematica v8.0.

The process of fixation and the dependence of ρ with the mutation rate μ have been described in detail elsewhere [ 9].

Significantly mutated genes (SMGs) were defined as the somatic mutations with higher mutated frequency than background mutation rate.

More importantly, due to this distinct reprogramming intermediate stage, it will be of great interest to characterize the iPSCs obtained with this method in details, e.g. the mutation rate arising during reprogramming and the epigenetic memory retention.

Obviously, these processes depend on the parameters of the system, in particular on the mutation rate μ, as will be discussed in detail below.

As in the ovarian case study, YS is biased toward identifying genes in the high background mutation rate categories (see Supplementary Section S5 for details).

A theoretical basis and further details of the adaptive mutation rate operator is included in the Theory section under the Discussion.

This estimate of N e is heavily dependent on the genome-wide mutation rate in Populus and this has not been characterized in great detail [ 33].

In detail, smokers featuring⩽15 pack years had a three-fold higher EGFR mutation rate than those with>15 pack years (12% vs 3.9% NS).