Sentence examples for mutation proportion we from inspiring English sources

After correcting for the false mutation proportion, we explored mutation calling using three different thresholds for optimization (Additional file 1: Table S1).

Thus, we explored the use of a false mutation proportion subtraction methodology that we developed in-house.

For each mutation detected, we divided the true mutation proportion by the expected mutation proportion for a single mutation (threshold) to estimate the number of copies of that mutation in any given sample.

The data were then normalized by subtracting the false mutation proportion from the total mutation proportion to determine the true mutation proportion at each nucleotide position (Additional file 1: Figure S2; see glossary for clarification).

Due to homopolymer sequencing errors, the false mutation proportion for some insertions/deletions (indels) can be much higher than false mutation proportions for base substitutions.

False mutation proportion refers to the sequencing artefacts that occur by chance at each nucleotide position.

Thus, the false mutation proportion subtraction accounted for many sequencing artefacts occurring around homopolymer regions.

Thus, indels were only called if the false mutation proportion was less than 2.15 × 10−2.

First, our method uses a simple false mutation proportion subtraction to minimize sequencing artefacts and alignment errors.

Indels occurring at sites with a false mutation proportion greater than this were not included in the analysis.

However, these false mutation calls are consistent across samples (Additional file 1: Figure S1) and thus the false mutation proportion of each mutation type can be estimated individually at each nucleotide position.