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Targeted mutation profiling was performed using the Sequenom Massarray 4 platform (Sequenom, San Diego, CA).
Somatic mutation profiling was performed using Sequenom LungCarta v1.0, and correlated with clinical parameters.
Somatic mutation profiling was accomplished by using the Sequenom LungCarta v1.0 panel (http://www.sequenom.com) (Sequenom, San Diego, CA).
Mutation profiling was repeated on 32 mutant samples using ColoCarta and the results were identical to results with OncoCarta, demonstrating that this methodology was reproducible.
Lymph node metastases were not routinely collected in C-07 but as a pilot study to determine the feasibility of using lymph nodes for mutation profiling was conducted.
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Tripathy, D, Harnden, K, Blackwell, K, and Robson, M. "Next generation sequencing and tumor mutation profiling: are we ready for routine use in the oncology clinic?." BMC medicine 12 (January 2014): 140-.
In this regard, mass spectrometry based mutation profiling is a robust technique that can be applied on frozen or formalin-fixed embedded tissue, and is particularly well-suited for multiplexed screening of hotspot alterations in large patient cohorts [ 40].
On the other hand, the K-ras mutation profile was not related to the level of 8-OH-dG.
Otherwise it was named "SHV-like" and its mutation profile was stored.
Otherwise, a new protein entry was created and its mutation profile was stored.
Subsequently, the mutation profile was matched against those mutation profiles listed in the TEM mutation table for the already assigned TEM β-lactamase sequences.
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