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Additionally, a Q672K mutation produced a dramatic slowing of activation kinetics, a phenomenon also observed when mutating the corresponding residue in mammalian TMEM16F (Yang et al., 2012).
Thus, the mutation of Ghd10 did not affect the growth rate of the mutant, but the mutation produced a gain-of-function in inflorescence development.
Further experiments revealed that each mutation produced a more stable version of the energy metabolism protein, with the winner making the most stable one.
On average, an elo null mutation produced a 40% decrease in survival compared to wild-type.
The R225Q mutation produced a fully dominant protein [7] with increased AMPK activity.
Each mutation produced a complete or nearly complete Bah phenotype, with no more than 2% of animals acquiring biofilms (Figure 5).
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"If it were not the case, then every time a mutation produced an improvement in body plan — a large significant change in anatomy — the animal would die and the beneficial mutation would be lost".
The L532P mutation produced an ~ 5-fold increase in the IC50 for dronedarone inhibition of IhERG.
Then with the help of selection, crossover, and mutation produces a new population.
A 4 base pair deletion and a point mutation producing a stop codon were identified in 1 cell line.
The Gly28 Ser mutation produces a dramatic four-fold increase in its kcat and a remarkable increase in its stability.
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