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As a result, with those mutation presented in reporter (∆209 231, ∆207 232), luciferase expression failed to be regulated by miR-875 and miR-3144 (Fig. 2).
Without pre-selection each mutation presented in at least one patient is considered with a different weight.
The two techniques do not lead to a selection of mutations but to a different weighting of each mutation presented in the dataset.
This RHC mutation is similar to the hill-climbing mutation presented in the previous section but it has the following distinctive features: (i) only one chromosome is being mutated and when no further improvement is obtained by pull moves the procedure stops; and (ii) at each hill-climbing iteration, only one pull move transformation is applied for a position randomly selected.
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* Women with any of BRCA1 mutations presented in Table 2.
Furthermore, the sequencing revealed the presence of other different polymorphisms and mutations presented in Table 2.
Twenty-six patients (2.6, 95% CI 1.6 3.6%) were found to carry one of the four BRCA1 mutations presented in this study.
This may reflect the impact of the different Complex I subunit mutations present in these cell lines, or may have other as yet unknown (genetic) causes.
Furthermore, massively parallel sequencing robustly and accurately identified all mutations present in the dataset.
To determine the mutations present in each clone, it was necessary to de-multiplex the samples.
Third, beneficial mutations present in different copies, or lineages, can be brought together.
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