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DOI: http://dx.doi.org/10.7554/eLife.02725.026 10.7554/eLiFigure25.027 Figure 4 figure supplement 5. Mutation patterns obtained from MLH1-deficient, MSH2-deficient, and MSH6-deficient exomes.
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To explore the somatic mutation patterns, we obtained the somatic mutations from Supplementary Table 2 published by one Pan-Cancer analysis of TCGA project [ 23].
The increase in the repertoire of alternative flux patterns obtained in this way makes more likely that the detrimental effects of mutations could be neutralized.
We can estimate the existence of mutation and structural alterations in plant DNA after impact of different stressful factors on the bases of DNA patterns obtained after RAPD with the set of primers.
Figure 1 shows the AFLP patterns obtained.
Furthermore, patterns obtained in different laboratories are difficult to compare.
The patterns obtained accord with those previously described.
From the mutation patterns and mutation rates obtained for individual microsatellite markers, we provide guidelines for the properties of microsatellite markers to be useful for divergence time estimates in future genome-wide sequencing projects (to follow in this section).
Earlier studies have compared closely related strains to obtain mutation patterns based on the hypothesis that these bacterial strains had diverged so recently that selection will not have had enough time to play its role.
Finally, a D516Y substitution was detected in association with an unexpected mutation, G523W, not tiled on the DNA probe array, but which could be detected by analysing the hybridisation pattern obtained with the wild-type probes covering codon 523.
The presence of the desired mutation in the CCTα gene of the transformed and rescued CCTα-KO cells was confirmed by PCR, using standard conditions, and analysis of the pattern obtained by restriction enzyme hydrolysis of the PCR products.
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