While we also acknowledge the occurrence of artifactual changes, the E547K mutation has not been detected in over 300 FFPE tumour samples we have screened to date (unpublished data) and thus, we feel that this mutation may be particular to a subset of MBC.
Considering that polymorphisms in the HIV PR and RT genes are common, even among therapy-naïve patients, it could happen in rare cases that an individual primer on the microarray may not work for an individual patient even with an optimised set of oligonucleotide primers because of the presence of a mutation that is particular to this patient.
These results suggest that strand-specific mutation biases that are indicated by excessive G → T mutations occurring on the virion-strand (p-value < 0.051 in the case of both MSV and TYLCV) are probably conserved throughout the geminiviruses, and other biased mutations such as excessive G → A, A → G and C → T mutations may be particular to certain species or genera.
The chaperone-like role of NTD125 both in vitro and in cells raises the possibility whether WT p53 might possess a self-chaperoning role as un-cleaved molecule; intra-molecular chaperone-like fragments occur frequently in proteins and such proteins would be prone to changing conditions and in particular, to mutations in the critical building block region [49].
Conceivably, this may even have allowed the diploid organisms to "lower their guard", i.e. to reduce the fidelity of the replication of their DNA, and favour mechanisms of recombination [ 32], thereby favouring the appearance of novel adaptive mutations, helping them in particular to combat pathogens more efficiently, or to adapt to new environments.
Our studies were initially driven by the hypothesis that in lung cancer cell lines, tyrosine phosphorylation patterns would be related to the state of EGFR signaling, and in particular to EGFR mutation status.
Transposable elements (TEs) are known to be major contributors to genome variability and, in particular, to somatic mutations.
Transposable elements are known to be major contributors to genome variability and, in particular, to somatic mutations.
TEs are known to be the major contributors to genome variability and, in particular, to somatic mutations.
