Sentence examples for mutation of putative from inspiring English sources

The mutation of putative acid/base and nucleophile of the active sites of retaining glycosyl hydrolases, together with kinetic analysis of the mutants, and stereochemical identification of products lead to useful information for the understanding of the reaction mechanism of these enzymes.

Firstly, mutation of putative branch points A and B did not affect splicing.

Mutation of HRE (−1316/−1310) in the CXCR4 promoter blocked additive responses to TGF-β and hypoxia, while mutation of putative SBEs had little or no effect (Figure 3C).

Mutation of putative PAK1 serine phosphorylation sites did not prevent PAK1 phosphorylation.

Similar to ERp72, the mutation of putative active sites in Creld2 allowed the formation of a number of higher order complexes containing Creld2-V5 (Fig.  4C, left panel).

Conversely, Mutation of putative NLC1-C binding site in the miR-320/SGCZ (miR-383) promoter region recued the luciferase activity repression induced by Nucleolin and NLC1-C.

It is normally performed through electrophoretic mobility shift assays, DNAse footprinting, ChIP-to-chip assays, or mutations of putative TFBSs [ 13- 15].

Quick-change II XL Site-Directed Mutagenesis kit (Stratagene) was used according to the manufacturer's instructions to generate the mutations of putative PKC phosphorylation sites within the regulatory C-terminal domain of HCN2: T549A, T566A, T566A, S818A.

Mutation of two putative phosphorylation sites in the N-terminus of KCNQ1 and one site in KCNE1 (S102) blocked the inhibition of Ang II.

One outstanding question about the z3 phenotype is how mutation of a putative citrate transporter produces variegated leaves, although the all cells in the z3 mutant have the same mutant genotype.

We also tested whether deletion or mutation of the putative NES/NLS motif prevents PINCH1/WT1 interaction in podocytes.