Sentence examples for mutation of nuc from inspiring English sources

More importantly, concomitant mutation of nuc in a sarA mutant UAMS-14777) enhanced biofilm formation by comparison to the sarA mutant (p<0.001) (Fig. 7).

Phenotypic assays confirmed that mutation of sarA increased nuclease production and that it was completely abolished by mutation of nuc even in a sarA mutant (Fig. 6).

The production of active extracellular nuclease does require protease processing [48], but if this were a defining characteristic of the biofilm-deficient phenotype of a sarA mutant, then mutation of nuc and inclusion of protease inhibitors would not be expected to have a cumulative effect since mutation of nuc eliminates the production of nuclease in any form.

Indeed, we previously demonstrated that mutation of sarA in UAMS-1 resulted in increased production of nuclease and that mutation of nuc partially restored the ability of a UAMS-1 sarA mutant to form a biofilm [23].

Mutation of nuc in UAMS-1 UAMS-14711) had no impact on biofilm formation, but complementation of the nuc mutation with a plasmid-borne version of the nuc gene UAMS-15522) did limit biofilm formation to a significant degree (p = 0.001) (Fig. 7), presumably because it resulted in increased production of nuclease by comparison to the parent strain (Fig. 6).

In contrast, concomitant mutation of sarA and nuc significantly enhanced biofilm formation by comparison to the sarA mutant.

Attempts to improve the response by administering two different NUC or a combination of NUC and PegIFN-α have not provided a dramatic increase in the rate of functional cure.

Pre-treatment of Nuc with the anti-Nuc polyclonal antibody prevented the increase in PGE2 production (Fig. 6B), pointing to a specific implication of Nuc in this process.

Expression of Nuc in neutrophils was first assessed.

These results, showing enhancing impact of Nuc on COX-2-dependent PGE2 production, support the concept of a physiologically significant interaction between Nuc and COX-2.

It remains possible that lower levels of Nuc could be found elsewhere, but the thorough approach undertaken herein strongly points to a Golgi/ER localization for Nuc.