Sentence examples for mutation of lysine from inspiring English sources

To this end, gene expression changes upon mutation of lysine 37 were assessed by microarray analysis.

For example, the mutation of lysine to glutamine in eIF-4A protein resulted in severely reduced ATP binding, and complete loss of ATPase activity [24].

As MS analysis has revealed that H2BK37 dimethylation is a relatively abundant modification, we reasoned that mutation of lysine 37 would likely cause pleiotropic effects.

The mutation of lysine 41 to glutamic acid in SCR 1 of CR2 (K41E CR2) has previously been shown to abrogate binding to C3d.

We were therefore interested in determining whether mutation of lysine 762 which disrupts ligand-induced ubiquitination of the G-CSFR had any effect on Stat3 and Stat5 activation.

Mutation of lysine 104 to arginine, did not dramatically compromise Ras1 sumoylation (Figure 3F), suggesting that Ras1 contains multiple non-consensus sumoylation sites.

To be sure that the mutation of lysines in Δ5KA does not alter the overall protein structure, we further examined the secondary and tertiary structures of the WT and Δ5KA mutant using purified recombinant proteins.

As shown in Figure 3B, alteration of lysines 278 and 280 to alanine effectively abolished the activity of this construct, whereas mutation of lysines amino terminal to the SIM had little or no effect (compare lane 17 with lanes 2, 4 and 8).

As depicted in Fig. 3C, truncation of the nine N-terminal amino acids (Δ N9, left panel), as well as specific mutation of lysines 13 16 (m13 16, right panel), had no effect on the ability of L22 to bind EBER-1 RNA.

In interphase egg extract, mutation of lysines approximately doubled Ngn3 protein half-life (Ngn3 14.8±1.5 minutes, Ngn3KO 32.4±4.2 minutes) (Fig. 3A C).

These experiments do, however, show evidence of ubiquitylation on sites other than lysines, cysteines, and the N-terminus (most likely on serines/threonines) of Ngn3 leading to degradation, as the mutation of lysines, cysteines, and N-terminus (resulting in AcNgn3KOCO) is less stabilising than the addition of proteasome inhibitor Mg132 (Figs 1, 2).