Mutation of all four acceptor lysine residues [K79, K152, K256, and K799] inhibits SUMOylation.
On the other hand, mutation of all four lysine residues to arginine (G9a4KR) abrogated SUMOylation even in the presence of SUMO1 (Fig. 1f).
Mutation of all the Akt phosphorylation sites into alanine promotes nuclear localization of FoxO1 thereby promoting constitutive activation FoxO1-CA 66. FoxO1-CA 6
The most successful genetic mutation of all time has permitted the self-awareness that allows human beings to "empathise, guess, manipulate, out-think, out-fight – and, where necessary, co-operate".
Each mutation attenuated the reduction of luciferase activity by miR-124 (Fig. 5G), while mutation of all three binding sites resulted in abolished repression in luciferase activity (Fig. 5G).
The computational efficiency of our framework was demonstrated with the full-sequence design of the peptide with flexible backbone templates, corresponding to the mutation of all positions except the native cysteines.
These mice carry point mutations in the dystrophin gene and null mutation of all utrophin genes.
Finally, we show that mutation of all identified phosphorylation sites does not cause observable defects in growth rate or morphology.
Fig. 6C and 6D confirmed that mutation of all tyrosines did not affect GH-induced GHR endocytosis.
Mutation of all residues in either of these conserved sequences undoubtedly prevents correct folding of this domain.
Mutation of all of these sites in combination reduced the binding of NEDD1 and γ-tubulin, although it was not abolished [9].
