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ORFs of 341 pF1K clones were PCR-cloned using PrimeStar HS or KOD plus high-fidelity DNA polymerases, as described in Section 2. All ORF clones obtained by PCR cloning were sequence-verified and we found that the PCR mutation occurrence rate was less than 5 × 10−5 mutations per residue under the conditions employed in this study.
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Similarly, a higher rate of DNA mutation occurrence due to oxidative damage is found in rats undergoing psychological distress [ 109].
This gives an estimate of the spontaneous occurrence rate.
Their occurrence rate was reported numerically.
Bigger breasts have higher cancer occurrence rate.
Position enrichment is measured using this normalized mutation occurrence.
Throughout the study, we carefully disentangle the predicted effects of the mutations from their bare occurrence rates, e.g., by comparing to null models conditioned to the same number of missense mutations.
In order to estimate mutation rates for MRI regions versus their respective control regions, we counted the occurrence rates for rare SNPs.
In the mitochondrial D-loop sequences, we find several single step mutational derivatives of one of the major mitochondrial haplotypes, suggesting an unusually high mutation rate, or the occurrence of selective sweeps in mitochondria.
It is possible that reduced but not abolished MMR functionality caused by the partial loss of MLH1 expression may give rise to an intermediate mutation rate and the onset of cancer due to increased occurrence of somatic mutations.
The higher mutation rate in cancer may result from the occurrence of accelerated mutagenesis after the dysfunction of the DNA replication and repair systems that is common in many types of cancer.
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