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In view of the clearly defined phenotype of the His C mutation obtained here, these earlier observations might be due to incomplete deprivation of the newly synthesized histones or off-target effects, highlighting the value of the newly established mutation.
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The types of mutations obtained here are similar to those observed in soybean and other plants obtained with ZFNs [ 15, 21], TALENs [ 17, 18, 30] and CRISPRs [ 31, 33- 35]; small deletions were the most frequent mutations; SNPs were less common (Additional file 2).
Table 2 shows the mutation frequencies obtained here and from the COSMIC (Catalog of Somatic Mutations in Cancer) database [ 21].
The improvement of myotonia by flecainide obtained here suggests that there may be a relationship between the G1306E mutation and the response to flecainide therapy.
These high values obtained here support the possibility to detect and quantify heteroplasmic point mutations with high accuracy (for detailed analysis see Table 1S).
The Mathematica program can be obtained here.
The movie can be obtained here.
The DVD can be obtained here.
A copy of the brief was obtained here today.
for saliva magnesium and calcium concentrations, respectively, were obtained here.
Funding isn't particularly easily obtained here.
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