Sentence examples for mutation obtained by from inspiring English sources
MTHFR genotypes were identified by seeing the presence or absence of 677C→T mutation obtained by PCR followed by Hinfl restriction digestion.
In our previous study [ 24], the corresponding reciprocal L725V mutation obtained by site-directed mutagenesis of PaTPS-Iso resulted in the formation of sandaracopimaradiene as a minor product.
dsp1 1 is a null allele of dsp1, which is maintained as a homozygous strain [ 37]. corto, corto 07128 and corto L1 are loss-of-function alleles: a deficiency, a P-element insertion and an uncharacterized mutation obtained by EMS mutagenesis, respectively.
As described above, r = 1,250 for loss-of-function mutations obtained by EMS mutagenesis.
Interestingly, mutations in such residues did not change the effector spectrum of HbpR and neither did the other generated mutations, whereas previous mutations obtained by directed evolution of HbpR that did change the effector spectrum from 2-HBP to 2-chlorobiphenyl mapped in completely different areas of the A-domain (Text S1).
This double mutation-based 'calcium auxotrophy' allows direct selection of suppressor mutations as well as testing of potential suppressor mutations obtained by reverse genetics.
We characterized the impact of mutations in the LOV2 domain described in the literature as well as mutations obtained by random mutagenesis.
Bacteriophage resolvases and single-stranded nucleases are used commonly in the assay but have not been compared side-by-side on mutations obtained by engineered nucleases.
We present here the first comparison of the sensitivity of T7E1 and Surveyor EMC assays on deletions and point mutations obtained by ZFNs in frog embryos.
We present the first comparison of the sensitivity of T7E1 and Surveyor EMC assays on deletions and point mutations obtained by zinc finger nuclease targeting in frog embryos.
This screening included mutations obtained by NGS of 1.5 Mb of the MSY, plus others known to lie outside the sequenced regions, several of which poorly characterized at the population level.
