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If this is not the case, a new set of primers for the second mutation needs to be designed and used.
This technology has wide_ranging therapeutic potential and could lay the groundwork for treating diseases where a gene's expression needs to be altered (such as turning down CCR5 in HIV), or where a mutation needs to be repaired (such as sickle cell diseases or hemophilia).
The main advantage of this procedure is its rapidity as mutants can be easily obtained in less than a week if the 3-step PCR procedure is used, or in less than three days if the mutation needs to be transferred from one strain to another.
The mechanism for how α3 connexin leads to a dosage-dependent inhibition of nuclear cataract induced by γB-crystallin S11R mutation needs to be further investigated.
The significance of this mutation needs to be evaluated.
For machine learning prediction of stability changes, each mutation needs to be encoded with a number of predictive features.
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Each mutation needed to be on a different copy of the NPHS2 gene (in trans) to be deemed causative.
The simulation started with a singly mutant cell lacking the second mutation needed to become fully Lac+.
In addition, intra-tumor heterogeneity with the presence of clones of tumor cells lacking BRAF mutations needs to be considered, since wildtype BRAF can be activated by inhibitors designed to target mutated BRAF.
A standardised method of detecting EGFR mutations needs to be established as soon as possible.
It is dedicated to characterizing variants in clinical settings where only a limited set of relevant mutations needs to be quickly characterized with the highest possible accuracy.
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