Sentence examples for mutation mismatch assay from inspiring English sources

The T7 Endonuclease I (T7E1) mutation mismatch assay is commonly used to detect high indel frequencies (>2%–5%).

To facilitate rapid detection of gyrA and parC mutations, two mismatch amplification mutation assays (MAMA-PCR) were developed and validated in this study.

*Melt-MAMA, melt-mismatch amplification mutation assay; SNP, single nucleotide polymorphism; con, concentration; Tm, melting temperature for ancestral and derived Melt-MAMA amplification products.

Double-mismatch amplification mutation assay (DMAMA -PCR revealeDMAMA -PCRholerevealedins thepossess the B-7 allele of ctx subunit B. DNA sequencing of tcpA reV.aled a point mutation at amino acholeraetion 64 (N → strainse toe ctxAB possessr revealed four copies of the tandem heptamer repeat sequence 5 '-TTTTGAT-3 '.

All V. cholerae O1 strains isolated from both clinical and environmental sources (n = 28), including the O395 (CL), N16961 (ET) and 2010EL-1786 (Haiti variant, ctxB genotype 7) were analyzed using double-mismatch amplification mutation assay (DMAMA -PCR technique to DMAMA -PCRtechnique genotope.

Two mismatch amplification mutation (MAMA) assays were developed and used to facilitate rapid detection of gyrA and parC mutations.

In this study, we calculated the mutation rates across the entire genome by using haploid wild-type and mismatch repair defective cells in a mutation accumulation assay over ~170 generations.

A rapid and reliable mutation detection assay has been developed.

For this purpose, PCR amplicons representing selected loci can be screened for mutations by mismatch-detecting assays [ 1] or by high throughput sequencing [ 2, 3].

Ames reverse mutation assay.

A heteroduplex mismatch cleavage assay based on mismatch-specific endonuclease Cel I is the most established method to detect point mutations [ 11, 21], but in this method the reaction conditions depend on the target region [ 22].