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We observed that Ba/F3-EpoR cells expressing JAK2 V617F, JAK2 K539L, JAK2 T875N and JAK2 R683G could proliferate in this medium, while the same mutants with the additional F595A mutation lost most of their autonomous growth (Figure 4B).
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In theory, the backcrossed congenic mutant strain should have lost most of the other ENU-induced mutations and SNPs derived from DBA/2 J; however, the rate of SNP and mutant loss per generation has not been examined experimentally.
He lost most of his fortune.
He apparently lost most of hers.
Mars has lost most of its oxygen.
We've lost most of our divers!
He lost most of his right leg.
However, he quickly lost most of them.
Who stands to lose most?
They could lose most of their money.
While R57 and R56 are able to remodel 7SK, the R52A mutation loses this capability.
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