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The Q77R amino acid substitution was constructed using PCR amplification with primers encoding the mutation listed in Table 4.
The primers are used for mutation listed in Table 2. Disruption of dr0615 did not show a growth defect (data not shown).
We intercrossed the G0 founders to produce hemizygous males (Il2rg−/Y) and homozygous females (Il2rg−/Il2rg−) for the ZFN-induced mutation listed in Table S3 to characterize the immunodeficient phenotypes of the X-SCID rats.
Complementary pairs of synthetic oligonucleotide primers containing the desired mutation (listed in Table 1) were used to amplify the full-length plasmid with the AQP1 cDNA insert.
The percentages of subjects that had at least one mutation listed in the TPV mutation score were not significantly different in both groups (11/21 vs. 15/20, P = 0.197).
Clinically significant genotypic resistance was defined as HIV viral load >1000 copies/mL and presence of ≥1 drug resistance mutation listed in the Spring 2008 update from the International AIDS Society [ 21].
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aOne mutation listing in the Breast Cancer Information Core database as unclassified variant (UV) encodes a nonsense mutation.
The fragments containing either of the mutations listed in the table were inserted into the expression vector, pMAQΔc2.
JEV SA14 and SA14-14-2 ectodomainsctodomares are distinguished by seven mutations (listed in the Table S1).
In addition, a total number of 142 disease-associated mutations were found in those top hCM-enriched genes (mutations listed in Fig. 4B, references listed in Table S14), suggesting a preferred enrichment of disease causing mutations in hCM-specific genes responsible for critical human heart functions.
Putative kinase domain mutations listed in the manual report were subsequently verified at MSKCC by bidirectional sequence analysis of a separate individual PCR product.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com