Sentence examples for mutation library achieved from inspiring English sources

When compared to KvarQ [ 35] the mutation library achieved higher sensitivity for resistance to isoniazid, pyrazinamide, ofloxacin and amikacin with increases of 5.9%, 8.2%, 3.5% and 7.9%, respectively, without compromising specificity (Additional file 1: Table S5).

M.P., B.A.P., and C.S. designed and created the TAS2R16 mutation library.

A randomized mutation library of the crtI gene in pLM495 was generated with a mutation rate of ~5 10 bp per kb.

(a) Each of the four TAS2R16 ligands selected was tested against the entire TAS2R16 mutation library (573 individual mutations, n = 2 3) and values derived for each are compared.

Here we used a comprehensive mutation library of human TAS2R16 to map its interactions with existing and novel agonists.

The mutation library and controls were expressed in HEK-293T cells in 384-well plates as described21.

Concentrations for mutation library screening were chosen to be approximately 2 to 3 times higher than the EC50 values for the ligands to maximize the sensitivity of detecting decreases in signaling due to the mutation in each clone.

(a) Calcium traces obtained from a representative 384-well plate of HEK-293T cells transfected with the TAS2R16 mutation library and stimulated with 3 mM salicin.

Each construct in the mutation library was tested for full-length TAS2R16 translation and surface expression by immunofluorescent antibody binding assays.

These data indicate that the mutation library was successfully constructed.

Other mutants (G276K, G276Y, and G276N) at this site were also screened from the saturated mutation library and exhibited better catalytic properties compared to the wild type.