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Concern on the disruption of the scFv which affects the binding activity due to the mutation is subject to further investigation.
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After drug treatment, the secondary mutation is subjected to selection and the cells with secondary mutation become a majority of the tumor.
However, the cost of profiling a cancer genome at base pair resolution remains out of range for routine clinical use, and calling mutations is subject to ambiguities as a result of tumor heterogeneity, when DNA is obtained from bulk tumor tissue.
Control and Mnn1 null embryos and larvae heterozygous for the mwh1 mutation were subjected to repeated but short 20 min heat shock treatments at 37°C.
Women in families without a demonstrable BRCA mutation were subjected to annual mammography.
Women from breast cancer families without a demonstrable BRCA1/2 mutation were subjected to annual mammography from age 30 years onwards.
Women at increased breast cancer risk from families without a demonstrable BRCA1/2 mutation were subjected to annual mammography from age 30 35 years onwards.
One μl (~150 ng) from six different DNA samples that had the desired mutation was subjected to PCR amplification employing primers for the human ε- and γ-globin genes.
Each laboratory-induced mutation was subjected to Condel, PolyPhen-2 and SIFT analyses using their respective web servers (bg.upf.edu/condel/analysis; genetics.bwh.harvard.edu/pph2 and sift.jcvi.org).jcvi.org
We obtained DNA oligomer frequencies at SNPs and DIMs and in their corresponding reference regions to address whether both types of mutations were subject to similar mutational hotspots.
However, we show that BRCA1 mutations are subject to strong negative selection.
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