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Specifically, the authors should identify the specific mutation in the recipient strain and then show that this mutation is lost in the transductants.
In cells undergoing mitotic division the cells with high levels of mtDNA mutation appear to be at a disadvantage and the mtDNA mutation is lost during life [29].
Let h X, Y be the proportion of cases where the heteroplasmy persists and h X and h Y be the proportions where the mutation is lost or fixed.
First, the probability that a beneficial mutation is lost due to a deterministic evolutionary phenomenon such as CI depends partly on stochasticity from the time that other mutations of similar effect arise.
Thus, it is plausible that an inherited mutation is lost from the blood, but detected in the muscle, explaining why some inherited mutations are more likely to be detected in the muscle than in the blood.
The beneficial mutation is introduced into one randomly chosen individual in generation 0. If the beneficial mutation is lost due to random drift, the population is reset to the neutral sample and the beneficial mutation is introduced again until a successful sweep is observed.
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Among the 67,964 replicates retained, the mutation was lost before 1950 in 46,719 (68.7%) cases and manifested in 5563 (8.19%) cases.
Among the 34,001 replicates retained, the mutation was lost in 23,242 (68.4%) cases and manifested in 2787 (8.20%) cases.
Among the 320,000 simulations performed, the mutation was lost before 1940 in 182,221 (56.9%) cases and manifested in 26,328 (8.23%) cases.
The probability of a beneficial mutation being lost by drift is approximately 1 - 2 s, where s is its selective advantage [ 44, 53].
The lethal mutation was lost from 7 strains prior to mapping, identified by the loss of the GFP marker, which indicated balancer breakdown.
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