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Simulations of the model exhibit key characteristics found in the experiments, including a preference for follicle-stimulating hormone synthesis at low pulse frequencies and a loss of this characteristic when a mutation is introduced.
Typically, an identical mutation is introduced into S. Typhimurium and the virulence of the strain is determined by oral administration to mice.
During the repair of one lesion, a mutation is introduced.
Instead, both r1 and r2 are decreased when the Y254V mutation is introduced.
The query mutation is introduced into a pool of heterozygote diploid knockout yeast strains by integrative transformation.
In the second method, random domain-localized (RDL) mutagenesis, a mutation is introduced at random within a specific domain of a gene.
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In brief, the S89G mutation was introduced in exon 6 using an overlap extension-PCR method.
Subsequently, a R419L mutation was introduced into purA to reduce the flux from IMP to AMP.
The R217Q mutation was introduced by site-directed mutagenesis.
The AXXLL mutation was introduced by a fusion PCR approach.
The point mutation was introduced with the QuickChange XL Site-Directed Mutagenesis kit (Stratagene).
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