Meanwhile, clonal operator and Cauchy mutation are integrated into the proposed algorithm to avoid premature convergence.
To verify that R3590I is responsible for the suppression, the mutation was integrated into CY4398 and a haploid spore colony isolated after sporulation.
The yMLH1-G64E mutation was integrated into the yeast genome by two-step gene replacement, as described previously (Hoffmann et al, 2003).
As shown in Author response image 1, the performances as judged by ROC analyses were found similar whether TP53 mutation was integrated into the prediction (as determined either by all exon sequencing [blue curve] or only exon 5-8 sequencing [green curve]) or not (red curve).
The pdc2Δ344 and pdc2Δ519 mutations were integrated into the yeast genome through homologous recombination following the method described by Güldener et al. (1996).
A solution to this dilemma is to include in the mutation cassette an extra fragment of the target gene that restores an open reading frame when the mutation cassette is integrated into the genome.
Inclusion of a segment of the essential gene consisting of synonymous codons restores an open reading frame when the mutation cassette is integrated into the genome and decreases the frequency of recombination events that fail to incorporate the desired mutation.
The mutation operator is integrated to promote the children generation diversity.
The SNP and CA repeat data is integrated with the mutation data to provide a wider genetic perspective of these samples.
At some point in the process, mutations arise — perhaps when the cDNA is integrated into the genome, or at an earlier stage (not shown).
