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Failure of the QTL alleles to complement the candidate gene mutation is indicated by a significant cross (mutant versus wild-type allele of the candidate gene) by inbred line interaction term from analysis of variance of life span.
The HX mutation is indicated and highlighted in red.
(b) The number of isolates with the noted mutation is indicated in a given year.
An increased risk associated with mono-allelic MUTYH mutation is indicated, albeit small and not currently clinically relevant, and likely specific for the variant Y179C.
For mutations identified by sequencing of DNA from clones harboring PCR products, the number of clones harboring the same mutation is indicated in parentheses.
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Haplotypes from spouses who married into the family and do not contain the ALS-A mutation are indicated by grey bars.
In case a mutation was indicated, the complete open reading frame of PRNP was analyzed by direct sequencing of PCR products using the automated LICOR 4200 DNA analyzer as described previously [5].
PI3K mutation was indicated to be associated with ER positivity, HER2 negativity and primary tumor size, which were not observed in our study [ 37, 39, 40].
The borders of the ORF/3′ UTR and redefined ORF/3′ UTR upon PTC mutation are indicated by solid or dashed lines below the constructs.
The borders of the original ORF/3′ UTR and redefined ORF/3′ UTR upon PTC mutation are indicated by solid or dashed lines below the constructs.
Extended primers were separated by capillary electrophoresis in the Genetic Analyser 3500 Dx (Applied Biosystems), and the presence or absence of a mutation was indicated by the incorporated wildtype or mutant labelled dideoxy nucleotide.
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