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The efficiency of ACE is such that success is assured and, moreover, cannot suffer from false positives as the nature of the pyrE deletion is such that reversion of the mutation is impossible.
Similar(59)
Directionality of the mutation was impossible to determine in one case because of a 5 bp difference to both possible parental alleles.
While expanded alleles sizes within the range of full mutation were impossible to determine with the methodology employed, the alleles within the premutation range were successfully genotyped with a CGG repeats number varying from 55 to 104.
It is important to note that mutation accumulation inference methods only make predictions about mutations that have moderate fitness effects mutations with weak fitness effects are difficult to detect, and neutral mutations are impossible to detect.
It is important to note that although the diversity observed in the described strains seems to have originated from point mutations, it is impossible to dismiss the impact of recombination in the generation of genetic diversity.
60 Moreover, one might be interested in the proportion of mutations with S around 1, as this class of mutation might be impossible to select out and yet might be damaging to the mean fitness of populations.
With direct cloning it is impossible to distinguish between true mutations and mutations that occurred during replication of the plasmid.
Currently, it is impossible to predict how mutations in these motifs will affect the SRL structure.
It is impossible to predict whether these heteroplasmic mutations will become full mutations or if they will be lost in future generations.
If every baby has 50-150 new mutations, it's impossible for all 50-150 mutotions to be deleterious; that would cause the extinction of man.
They also said it is impossible to distinguish between spontaneously occurring mutations and those induced by human intervention, creating a major regulatory headache that could hamper trade.
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