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The AS160T649A knockin mouse, in which the tenth exon harbouring the T649A mutation is flanked by loxP-Cre excision sites, was previously generated in our laboratory [ 12].
We previously reported the generation of an AS160T649A knockin mouse in which the tenth exon harbouring a T649A mutation is flanked by loxP-Cre excision sites [ 12].
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Decisions on whether or not a mutation was flanked by two markers were based on whether recombinations with the markers occurred independently.
To accomplish this, the PB1 open reading frame, in which the terminal packaging signals were inactivated by serial synonymous mutations, was flanked by the NA segment-specific packaging sequences including the NCRs and the coding region packaging signals.
Because most of the target mutations were flanked by genetic markers that are already identified to the level of the gene sequence, the amount of the genome that require examination for each strain ranged from a low of 74 kilobases (kb) in strain FGSC 305 to over 4 megabases (Mb) in strain FGSC 3562 (Table 1).
Yeast surface display libraries were constructed and analyzed by flow cytometry to prove the hypothesis that the mutations Q418L and S424T are able to preserve the structural integrity of the domain after diversification and elongation of the loop region that is flanked by the mutations.
Albi is flanked by the Mediterranean and Atlantic Coasts.
The panel is flanked by door keepers.
We also found evidence for context-dependent mutation rates, with higher mutation rates for focal nucleotides that were flanked on either or both sides by a C G base pair.
47– 50 Most deletions are flanked by segmental duplications 51 and occur as de novo mutations mediated by nonallelic homologous recombination.
James was flanked by two security agents.
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