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By separating the luminal and basal cells for independent culture, we show that the effect of the Lrp5 null mutation is evident not only in the basal cell population (where we would anticipate the effect of this mutation), but also in the luminal cells.
The point mutation is evident in the regions of high amino acid similarity, because these regions diversify by changing codons and thus the amino acid identity, while likely retaining much if not all of the function of the regions.
Furthermore, protection from mutation is evident at the TBP binding site while the peak of mutations ∼30 base pairs downstream identifies the site of RNAP loading and DNA melting mapped by permanganate footprinting (Giardina and Lis, 1993) and high resolution ChIP (Rhee and Pugh, 2012).
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Consistent with previous published data [7], [44] we found that the BRAF V600E mutation was common in serrated polyps (11 out of 21) whereas the KRAS mutation was evident in only 4 out of 21.
The splice site mutation was evident as no wild-type sequence was present.
By contrast, the WRT64 66 mutant produced less relief of polyamine block than did γ-2 WT, where the effect of the mutation was evident only at positive potentials (data not shown).
A third distinct cluster of mutations is evident at ∼25% of reads, the equivalent of cluster C. All of these, as predicted, are phased with the retained copy of chromosome 13.
For example, in the absence of both mutY and mutM, thousands-fold increase in CG-to-AT mutations was observed, and the same magnitude of mutations is evident in mutT-deficient strains, but with an opposite mutation spectrum, namely AT-to-GC [ 37].
Considering that AZFc maps to the male-specific region of the Y chromosome (and is therefore bereft of homologous recombination with a chromosome partner), the functional relevance of this model as a counter to the accumulation of deleterious mutations is evident.
So the effect of the mutations was evident in unselected traits like acetate utilization.
These authors suggest that expression profiles for DNA damage response are more robust at toxic doses where mutations were evident.
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CEO of Professional Science Editing for Scientists @ prosciediting.com