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Once a mutation is confirmed, it is possible to screen family members for the disease as part of clinical genetics family counseling.
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The mutation was confirmed by sequencing.
The correct mutation was confirmed by PCR.
The presence of the desired mutation was confirmed by sequencing.
The threonine to alanine mutation was confirmed by sequencing.
The resulting mutation was confirmed by sequence analysis.
The introduction of the mutation was confirmed by sequencing.
Specific incorporation of the c.1114C>T mutation was confirmed by sequencing insertion-specific PCR products.
The mutation was confirmed in the DNA of all affected dogs in the study (n = 30).
The K220A mutation was confirmed by DNA sequencing (see Materials and Methods).
Each mutation was confirmed by DNA sequencing at the University of Pittsburgh Genomics Core Facility.
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