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Pollen phenotype is readily checked on the F1 progeny from crosses between the rpt5a-4 mutant (in a Col-0 background) and any other accession: 100% of the pollen should be viable if the rpt5a-4 mutatisn is complemented -by RPT5b x - but only 75% will be viable if there is no complementation (Fig. 2A and 2B).
Interestingly, the effect of this mutation is complemented by the findings with HERG (human ether-a-go-go related gene) K+ channels, where a structurally analogous, but reverse mutation, Y652F, suppressed the voltage-dependent block by quinidine (Sanchez-Chapula et al., 2003).
An ade2 ade3 elg1 Δ srs2 Δ diploid strain (OP738) kept alive by the presence of a LEU2 -marked plasmid carrying the ELG1 gene as well as the ADE3 gene forms uniformly red colonies (as the ade3 mutation is complemented, and any cell that loses the plasmid dies; Figure 2).
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These behavioral effects of Afh mutation were complemented by the specific disruption of the Per/Cry circadian regulatory complex in brain regions that govern food anticipatory behaviors, sleep, and timing.
The olsA mutation was complemented following the same protocol by introducing plasmid pYLI-1 into the BABΔolsA mutant.
Growth recovery by ers1 mutation was reversed when the ers1 mutation was complemented by ERS1p ERS1.
However, quantitative differences in noncomplementation can also result from background-dependent dominance of the mutation being complemented (Service 2004).
The mutation was complemented by transformation of the Ames 35 ΔAtxA strain with plasmid pIU68 [ 14], which contains the atxA gene and its endogenous promoter region.
We previously showed that a strong rpt5a-1 male gametophyte mutation was complemented by a transgene harbouring a 4 kb RPT5b Col construct [ 15].
When these mutations were complemented by expressing pilA or omcZ on a plasmid, wild type levels of current production were restored (Table 4).
To show that the nonacidifying phenotype of the D15 mutant was caused by the mutation in laeA, the D15 26 mutant was complemented with the laeA gene.
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