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Therefore, increased susceptibility to disease may accompany a fitness-increasing mutation introduced by positive selection.
Characterization of precise genetic mouse models with the equivalent mutation introduced by knock-in techniques into Hdh, the mouse HD ortholog, indicates that molecular differences are evident even at the embryonic stem (ES) cell stage [ 9, 10].
A base miscall in one of the BAC end reads, or even a mutation introduced by the E. coli replication machinery in the BAC sequence, could create a false SNP that is not present in the genomic sequence, as it was confirmed by direct Sanger sequencing of the genomic DNA amplified by PCR.
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Point-mutations were confirmed by restriction digestion of plasmid DNA with Eco 72 I for mutations introduced by pair #1, and Eco 147 I for mutations introduced by pair #2, as well as by sequencing.
We speculated that such decline in cell growth was caused by the random mutations introduced by GREACE.
Interestingly, the frequency of mutations introduced by gRNA complementary to the deletion mutant is significantly higher than with gRNA complementary to WT donor.
Parenthetically, mutations, introduced by gene targeting, might also be viewed as a form of stress.
The "mutations" introduced by virtue of modifying experimental protocol were very innovative and interesting.
The 120 nt changes introduced by conspecific LGT events are approximately three times the 38 (34 single mutations + 4 2x2 double mutations) introduced by mutation.
One quote from a student follows: The "mutations" introduced by virtue of modifying experimental protocol were very innovative and interesting.
We have demonstrated the usefulness of a single blastocyst-based assay for detecting mutations introduced by the CRISPR/Cas9 system.
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