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The generation of the S183/T185 double mutant occurred by introducing the T185 mutation into the S183-mutated cDNA.
To test the functional consequences of the mutated F2 in enhancer blocking we introduced this mutation into the F1F2 enhancer blocking construct.
A triple mutant where all three histidines were mutated to alanine was constructed by introducing the H306A mutation into the H252A/H232A double mutant.
So why not introduce that mutation into the human genome?
In such cases, the high prevalence of an unusual condition in a population can be traced to a founding ancestor who happened to carry a novel mutation into the region.
A nonintegrating mutant, SIVsmD116N, was derived from the infectious pathogenic SIVsmE543-3 clone by introducing an Asp (D) to Asn (N) mutation into the catalytic domain of integrase.
Other targeted modifications include placing genes under the control of heterologous regulatable or constitutive promoters, introducing epitope/protein tags to the target protein, or introducing a mutation into the target gene.
The additional introduction of the A243T mutation into the zwf gene and the over expression of fructose 1,6-bisphosphatase resulted in a further successive improvement of lysine production.
[1] The snopes article on kidney theft is good but incomplete -- it doesn't mention the baby parts antecedent in South and Central America and its likely mutation into the U.S. kidney theft tales.
Even when her wax lookalike debuted in 2005, Hilton's once-trendy tracksuit had begun its mutation into the comfort wear of the attraction's tourists and a uniform of the masses.
We therefore introduced the W187A mutation into the PR8 NS1 ED bacterial expression construct.
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