Sentence examples for mutation inserts were from inspiring English sources

To ascertain a leading or lagging strand preference for duplication mutations, several short DNA sequences, i.e. mutation inserts, were designed that should demonstrate an asymmetric propensity for duplication mutations in the two complementary DNA strands during replication.

Wild-type and mutation inserts were confirmed by DNA sequencing.

The plasmid inserts were sequenced completely to ensure that unwanted mutations during amplification and cloning had not occurred.

The PCR fragment was cut with the relevant enzymes and ligated into pET30a which contains a sequence encoding an N-terminal His tag; clones which displayed no mutation after full sequencing of the insert were used for further experiments.

Mutations in the metavinculin-specific insert are linked to heart disease such as dilated cardiomyopathies.

Nucleotide sequence further confirmed that the sequence of recombinant plasmid was correct without base mutation and deletion, and the sequence inserted was corrected by comparing with GenBank as is shown in Figure  2.

The insert was sequenced to verify that the intended mutation was correctly constructed.

The individuals from the youngest generation who had the mutation were inserted into the case dataset.

The insert was amplified by overlap extension PCR using overlapping primers introducing the 34L>34P mutation.

The presence of the insert was confirmed by PCR with primers oLC805 and oLC11, and by sequencing over the entire insert to ensure no mutations.

For each mutation, sequences spanning 27 nucleotides centered on the different stop mutations were inserted into the dual reporter vector pCRFL in-frame between Renilla and Firefly luciferase coding sequences [Floquet et al., 2011].