Here, we report the genome editing and targeted gene mutation in plants via the CRISPR Cas9 system.
The diversity in phylogenetic and genomics scale suggests that there is no simple rule or single mechanism underlying mutation rate variation in plant mitochondrial genomes.
Given the probability distribution of the nonlethal fitness effects of single mutations in plant viruses (Carrasco et al.
These results further strengthen the idea that plant resistance to rapamycin evolved as a consequence of mutations in plant FKBP proteins.
Recently, high resolution melting (HRM) analysis has succeeded to detect point mutations in plant populations [ 25], medaka TILLING library [ 26] and human disease [ 27].
To gain some insight into this, we utilized our structural and biochemical data to engineer mutations in plant Sgt1, in vivo, which would be expected to specifically disrupt its interaction with Hsp90, but not with other known ligand proteins.
The DASH method and DASHboard software can be used to carry out large-scale analyses of the compositional variation of plant cell walls and biomass, to compare plants with mutations in plant cell wall synthesis pathways, and to characterise novel carbohydrate active enzymes.
That the mitochondrial mutation rate has returned to normally low levels in many Geraniaceae raises the possibility that, akin to the ephemerality of mutator strains in bacteria, selection favors a low mutation rate in plant mitochondria.
The low mutation rates in plant mitochondria have been a challenge to explain (Lynch et al. 2006; Lynch 2007).
The variation in rates across genomic and phylogenetic scales raises questions about the mechanisms responsible for the evolution of mutation rates in plant mitochondrial genomes.
