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To characterize the ls-1 mutation in more detail, a cDNA coding for a pea KSA was cloned and shown to be encoded by the LS locus.
Therefore we identified the IDH1 mutation in more than 45.4% of glioblastomas.
In addition, this large-scale genetic study found no GIST with an activating mutation in more than one exon of the c- kit gene.
We can then estimate the number of genes expected to carry a causal mutation in more than one case as a function of N (Fig. 2).
There is very little positional overlap of mutations across samples, but 649 genes have a non-silent mutation in more than 10 samples.
The latter possibility is extremely unlikely given the absence of this mutation in more than 129,000 alleles studied to date.
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Four cases showed a single mutational event and 4 revealed several mutations in more than one exon.
To study these mutations in more detail, we then analyzed the mutated sequences in Micro-Tom using Sanger's method and next-generation sequencing (NGS) by Mi-seq (Fig. 2b,c).
While p53 contains inactivating mutations in more than half of HPV-negative oral cancers, it is rarely mutated in HPV-positive SCCHN [ 8].
Mutations in more than 40 genes have been identified to date in CMT.
The statistics of library with mutations in more than 20 amino acids can be calculated easily.
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