The U2AF1 mutation frequency was higher, with different frequencies in the mutated sites of U2AF1 (S34Y, 6/25; S34F, 11/25; and Q157P 8/25).
After irradiation with accelerated uranium ions (8.8 MeV/u, 15,580 keV μm−1) a significant decrease of mutation frequency was found with higher particle fluences (3 × 106 particles cm−2).
The overall mutation frequency was higher in this meta-analysis than the current study.
In the original Tangkou population, the L1014F mutation frequency was 71.7%.
This mutation frequency was dramatically elevated in chronologically aged cells ectopically expressing CLN3 (Fig. 5D; "pCLN3").
Without deltamethrin selection, the L1014F mutation frequency was reduced to 41.7% after 12 generations.
Thus, the mutation frequency was 2.5 fold less than for the first two exons of the RASSF1A (see above).
H2O2-induced mutation frequency was calculated by subtracting the spontaneous frequency of RifR from that observed following H2O2-treatment.
Mutation frequency was calculated as the ratio of 5-FOA-resistant colonies to the average number of colonies plated.
Mutation frequency was assessed by determining frequency of resistance to the toxic amino-acid analogue canavanine conferred by mutations in the CAN1 gene.
Spontaneous mutation frequency was calculated by dividing the number of RifR cells by the total number of viable cells in the culture.
