This frequency is comparable with the mutation frequency of BRCA1 (∼2%) in breast cancers (Kurian, 2010).
Control experiments through subcloning and sequencing confirmed that only the vector backbones were mutated with an average mutation frequency of ~1.25 mutations per kb.
The second most commonly mutated gene in PTNFL was TNFRSF14, with a mutation frequency of 29%, similar to that seen in limited-stage typical FL (p=0.35).
The most commonly mutated gene in PTNFL was MAP2K1, encoding MEK1, with a mutation frequency of 43%.
Here we report a low median exonic mutation frequency of 0.60 per Mb (0.48 nonsilent) and notably few recurrently mutated genes in these tumors.
μ A variable representing the background mutation frequency of the tissue.
Surprisingly, wild-type Cas9 yielded a mutation frequency of approximately 50%.
Inhibition of repair could result in an increase in the mutation frequency of the base damage.
However, the mutation frequency of the NC4 spores formed in space was similar to that of the ground control.
Surface residues are colored based on mutation frequency of deleterious missense mutations at the time of analysis in cBioPortal, for residue positions 758–1335.
Importantly, the high mutation frequency of the wild-type Cas9 plasmid was not merely due to homologous recombination of the plasmid with the genome.
