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The statistical significance was obtained using Wilcoxon test to test the difference between two groups of cancer gene mutation frequency from Module One and Module Two.
A two-way χ test was used to evaluate the mutation frequency from the in vitro DNA end joining assay.
Together, these K-Ras mutations and the B-Raf V600E mutation increase overall Ras pathway mutation frequency from 27.4 to 37.8%.
We also identified seven genes with significant mutation frequency from the Cancer Gene Census [ 15] list known to have somatic mutations in colorectal cancers including the top five CAN genes, NRAS and BRAF.
We have noted that treatment of Arabidopsis seed batches with the same concentration of mutagen can vary in mutation frequency from experiment to experiment, probably because of the effect of environmental conditions on the plant response.
Quantitative measurement of the repeat size heterogeneity in blood samples by SP-PCR showed a high level of variation, with the mutation frequency from 50% to over 90%, and an allele size distribution skewed towards larger alleles with a lower boundary, below which variant alleles are rare [ 57].
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For each template strand, the polymerase error frequency was calculated by subtracting the ssDNA background mutation frequencies from the observed pol β HSV- tk mutation frequencies.
For each template, the polymerase error frequency was calculated by subtracting the ssDNA background mutation frequencies from the observed pol β HSV-tk mutation frequencies.
Molecular spectra from these studies were reanalyzed after adjustment for reported mutation frequencies from the individual studies and the entire data set.
Similarly, deviation of observed mutation frequencies from their expected frequencies was detected using a two-tailed chi-square test as in van der Walt et al. [ 29] which explicitly accounts for differences in parental virus nucleotide compositions.
We determined the number of mutations in these sequences in comparison to the consensus sequence and we estimated the mutation frequencies from the total number of nucleotides sequenced using the procedure applied by [ 17, 44].
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