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Mutation frequency does not decrease linearly as intracellular selection approaches its maximal value; the curve results from the fact that mutant genomes that are immediately packaged are not subject to selection, while the contribution of rare, early mutants to average mutation frequency may be reduced by multiple rounds of intracellular selection.
Mutation frequency does not capture the distribution of mutations among the individual components of a mutant spectrum, and the Shannon entropy reaches the maximum possible value of 1 when all the sequences under study are different, independently of the mutational load.
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Similarly to germline variation, somatic mutation frequency did not correlate significantly with local interspecies conservation level or distance from stop codon or polyA site (|r|<0.1).
One primary tumor tested with both OncoMap and OncoScan demonstrated evidence of Y373C mutation using OncoMap, but the overall mutation frequency did not meet the threshold in OncoScan for a positive mutation.
Although we were unable to address whether the mutations introduced by the system can be reflected by phenotypes, we think this is highly probable, based on the mutation frequency done by TALENs (Treen et al. 2014).
For complex III and V, Danish and southern European populations showed a higher frequency of mutations in controls than in the 90+ group (P = 0.005 in Denmark and P = 0.048 in South Europe) (P = 0.0011 and P = 0.0023, respectively), while in the Finnish population, the mutation frequency did not differ between the 90+ and controls for both complexes.
Gene mutation frequencies did not differ significantly between clinical benefit and no clinical benefit groups.
Third, the reports of mutation frequencies do not commonly find associations with survival [ 33– 33].
TP53 and PIK3CA mutation frequencies did not differ significantly between epitypes.
Mutation frequencies did not significantly vary with template size or length of formalin fixation.
Mutation frequencies did not statistically significantly differ between breast cancer-only families with and without an autosomal dominant inheritance pattern of the disease (23.8 vs 16.1%; P=0.085).
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