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Additionally, it accurately determined dosage-dependent and drug-specific alterations in mutation frequency associated with mutagenic, antiviral compounds.
The data presented herein clearly demonstrate that polymerase alone may not account for the high mutation frequency associated with type 2 viruses in the tk mutagenesis assay.
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We show that, by depleting SETD2 or RAD51 HR proteins, DSB induction leads to significantly increased mutation frequencies associated with deletions within six cell cycles (5 days).
The highest mutation frequencies were associated with the A8 cMNRs in PTPN13 (22%) and PTPN21 (26%).
Moreover, a high mutation frequency was strongly associated with the performance of all methods, particularly in terms of recall, regardless of whether that method had been specifically designed as a mutation frequency detector (Supplementary Figs 5 and 6).
Clearly, the overall mutation frequency is closely associated with INDEL frequency.
To further characterize the mutation frequency and phenotypes associated with tubulin mutations, we studied a cohort of 60 foetal cases.
Analysis results show that degree is not associated with mutation frequency or gene length but is positively correlated with the number of exons (R = 0.13, P = 0.02, Spearman's correlation).
The embB306, embB497, and embB406 mutation patters were ranked as the top three in mutation frequency and were found to be associated with EMB resistance.
After grouping the two datasets we observed that the mutation frequency of the target genes was associated with large bowel carcinoma location (Table 2).
The QRDR mutations in gyrA and parC of the clinical QRSP (donor strains CT01147 and UAB169), the mutants (Phe/Phe and Phe/Tyr), and parent strain (EF3030) were sequenced to confirm the presence of QRDR mutations and because genetic transformation has been associated with increased mutation frequency (21,22).
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