Although some tissue specificity was evident for each of the four mutational mechanisms, the majority of all mutation frequencies were not accounted for by any of these mutational mechanisms.
After the flight, DNA replication and mutation frequencies were measured.
On/off target mutation frequencies were calculated using the following formula as previously described22:.
Relative mutation frequencies were analyzed using a reporter plasmid that allowed direct comparison of the methods.
Point mutation frequencies were measured in a BY4741 background.
Mutation frequencies were determined as previously described [56].
The mutation frequencies were slightly different between the age groups, Table 1.
Mutation frequencies were recorded as the number of rifampicin-resistant colonies per 109 viable cells, which were enumerated using the ampicillin-containing plates.
Eleven sequences were from normal tissues (four clones with one mutation) and MF = 0.1, i.e. mutation frequencies were statistically significantly different (P = 0.025).
Because mutation frequencies were enhanced in P. aeruginosa biofilm microcolonies, we examined in more detail whether mutation can influence microcolony growth and development.
Mutation frequencies were calculated as the ratio of the number of mutation events (both, templated as well as non-templated) to the total number of base pairs sequenced.
