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Comparison of mutation frequencies was carried out by two-tailed unpaired t-test.
The overall procedure of using nucleotide mutation frequencies to estimate codon mutation frequencies was first suggested by Deminoff et al. who argued that adequate statistical power could not be realized if only codon-triplet mutations were analyzed.
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After the flight, DNA replication and mutation frequencies were measured.
On/off target mutation frequencies were calculated using the following formula as previously described22:.
Relative mutation frequencies were analyzed using a reporter plasmid that allowed direct comparison of the methods.
A subsequent study further showed that both the number of off-target sites and mutation frequencies are decreased using tru-RGNs as detected by GUIDE-Seq19.
Off-target mutations are screened out in the same predicated off-target sites, while at 9 out of 15 off-target sites, the respective mutation frequencies are not significantly different between tru-RGNs and std-RGNs.
IgE mutation frequencies are primarily increased in children with impaired skin barrier conditions such as eczema, suggesting that IgE affinity maturation could provide a mechanistic link between epithelial barrier failure and allergy development.
Point mutation frequencies were measured in a BY4741 background.
Mutation frequencies were determined as previously described [56].
The mutation frequencies were slightly different between the age groups, Table 1.
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