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In subsequent experiments utilizing larger promoter constructs, the HRE4 mutation also led to a greater loss in activity than the HRE5 mutation (Figures 4C and 4D).
Importantly, acadesine also inhibited cell metabolism in imatinib-resistant K562 cells and in Ba/F3 cells carrying the BCR-ABL-T315I mutation (Figures 1B and Figure S1D).
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The interactions of compound 46 with these residues were lost upon mutation (Figure 6g-h).
The number of successfully genotyped controls varies according to the specific mutation (Figure 1).
Genotyping PCR demonstrated that eggs from mutant mice contained the Separase mutation (Figure 1D).
However, the early flowering of atjmj4-1 was fully suppressed by the ft-10 mutation (Figure 3F).
Ten independent cybrid clones retained homoplasmic loads of the m.15533A>G mutation (Figure 1B).
As expected, this mutation dramatically inhibited synthesis of BM2fluc similarly to that observed with the A1 mutation (Figure 8B).
This exogenous expression system also enabled the investigation of the conversion activity of moPrP harbouring a P101L mutation (Figure 4A).
Even so, they do serve as effective tools to assay the consequences of the Ala187Val mutation (Figure 2).
As expected, the response of each fusion to AHL in motility agar was eliminated by the sdiA mutation (Figure 6).
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