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Therefore, mutation efficiency is easily underestimated.
This mutation efficiency is ten-fold higher than the 3-7 % obtained with transcription-activator like effector nucleases (TALENs) in soybean hairy roots [ 30].
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Mutation efficiencies were variable in animals raised from TALEN-injected zygotes, including evidence of biallelic mutation rates.
Thus, using changes in protein expression levels in the context of different intron sequences to assay the effects of mutations on splicing efficiency is a valid approach.
Intriguingly, we found that HBB −28 (A>G) mutation repairing efficiency was about 20% in the constructed cell line and primary skin fibroblast cells.
Since this transporter is largely unexplored, the effect of the mutation on riboflavin transport efficiency is currently unknown.
This result highlights that antioxidant efficiency is greater in unaffected mutation carriers.
However, the observed mutation efficiency should be high enough to generate heritable mutations according to our experience in silkworm.
The efficiency is particularly obvious for large fragment mutations.
Translation efficiency is also targeted by PI3K signaling and modulated by mutation in tumor suppressors PTEN and TSC.
Further, sporulation efficiency is strikingly different for yeast strains with distinct mutations or genetic backgrounds.
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