In the meantime, we note that when we omit the dps-deleted strain from our analysis of the mean effect of secondary mutations, disruption type still explains a substantial, though marginally non-significant portion of overall variation (F1,9 = 3.663, P = 0.088).
Of note, in addition to SF3B1 mutations, disruptions of other aspects of RNA processing have been observed in CLL, including recurrent mutations in DDX3X and XPO1 [ 34], highlighting the importance of RNA processing in CLL.
Being a genetically well-defined and well-tractable organism that is amenable to gene mutations, disruptions, or gene marking as well as gene-dosage effects renders yeast attractive in the study of disease processes such as the regulation of eukaryotic cell division or the elucidation of events leading to apoptosis [ 1].
As a critical tumor suppressor, p53 is inactivated in human cancer cells by somatic gene mutation or disruption of pathways required for its activation.
This was based on the idea that suppressor mutation of disruption of essential genes would have stronger effect than that of non-essential genes in terms of restoration of rDNA transcription activity.
p53 is critical to suppress cancer development in humans, and the loss of wild-type p53 activity through direct somatic gene mutation or disruption of pathways important for p53 activation is required for cancer progression (Vousden and Prives, 2009).
Since Dicer is required for processing of almost all mature miRNAs, mutation or disruption of Dicer has been widely used as an approach to investigate the biological significance of miRNAs in various cell types including cardiomyocytes [17] [19], endothelial cells [20] [22], fibroblasts [23] and immune cells 24 26].
Effect of bgl2 mutation and disruption in T. reesei QM9414.
Therefore, it was clear that intracellular cellobiase activity was affected by bgl2 mutation or disruption.
